supplemental animations for paper

Supplemental material for the following published paper:
Analysis of synaptic bodies in the Sprague-Dawley rat pineal gland under extreme photoperiods
Holger Jastrow and Jörg Racke
Micron Volume 38, Issue 3: 237-251 (April 2007).

Corresponding author: PD Dr. med. Holger Jastrow
E-mail: info@drjastrow.de

To start the animations click on the images, please!
Color: green = synaptic bodies
A. different stereo motion pictures of a reconstructed field of synaptic bodies inside a Sprague-Dawley rat pinealocyte with a section as background
The animations demonstrate the three-dimensional morphology of 7 synaptic bodies (sbs). They all are ribbons, some are strongly bent, one seems to have resulted from two
fused sbs. In order not to show the structures as closely as possible to their true shape no interpolations or smoothing algorithms were applied. Two sbs look somewhat
disrupted due to fact that they were nearly sagittally cut in the original sections. All sbs are more or less bent in at least one direction and only two are completely contained in
the investigated tissue volume. Also note the deep invagination of the cell membrane close to the field and several clathrin coated vesicles. The oriented stack of original images
from which the reconstructions were done is shown in C.

The reconstructed field rotates along the X-axis (file size: 11.8 MB!)
The reconstructed field is seen from above and rotates along the Y-axis (file size: 11.8 MB!)
The reconstructed field is regarded from below and rotates along the Y-axis (file size: 11.8 MB!)
The reconstructed field is visible from an oblique angle and rotates along the Y-axis (file size: 10.4 MB!)

B. stereo animations demonstrating reconstructed synaptic bodies of the same field without background

The reconstructed field
is seen from above
and rotates along the Y-axis
(file size: 8.6 MB!)

The reconstructed field
is regarded from above
at a different angle
and rotates along the Y-axis
(file size: 8.6 MB!)

C. oriented stack of original micrographs used for reconstruction of the field visualized in A and B

1 2 3 4 5 6

7 8 9 10 11 (interpolated, original damaged) 12

D. stereo animation in 3D "true colour", i.e. original grey tone as in the micrographs, from another reconstructed area

Three plate-like SBs close to the membranes of two adjacent cells one of which clearly shows the pentalamellar structure of SBs that is only visible when they are exactly seen from the direction of their long axis, i.e., if they are positioned exactly at right angle to the section plane.

--> further information on: synaptic bodies, pineal gland
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