Holger Jastrow1, Dirk Schmanke2, Jörg Weinert3, Udo Jendrysiak3 and Lutz Vollrath1
1 Anatomisches Institut, Johannes Gutenberg-Universität
Mainz, Becherweg 13, D-55128 Mainz,
2 Zentrum für Datenverarbeitung,
J. Gutenberg-Universität, Bentzelweg 12, D-55128 Mainz,
3 Institut für Medizinische Statistik
und Dokumentation (IMSD), J. Gutenberg-Universität, Obere Zahlbacher
Str. 69, D-55101 Mainz
Synaptic bodies (SBs) are dynamic synaptic organelles of afferent synapses
of retina, inner ear, lateral line organ and pineal gland in vertebrates.
When investigated in a transmission electron microscope their electron-dense
rod-like, round or irregular profiles are surrounded by electron-lucent
vesicles. The three-dimensional structure of pineal SBs is not precisely
known. Pineal glands of two guinea-pigs (one kept under a LD cycle of 12:12
h; one kept in constant light for 8 weeks) were investigated. SBs were
reconstructed in three dimensions to visualise morphological changes in
constant light. Transmission electron microscope micrographs from up to
18 serial sections with a known distance (50 nm) of groups of SBs were
scanned and processed (controlled superimposition of corresponding organelles
from adjacent sections, involving rotation and shifting of the scanned
image) by a self - written programme in the Interactive Data Language (IDL®).
The resulting 3D-array was further processed by NeurOPS (neurosurgical
operation-planning and simulation software developed at the IMSD) allowing
3D visualisation of SBs. The three-dimensional images were printed from
different angles to elucidate the appearance of SBs in space. It was found
that under constant light SBs lay opposite to one another in adjacent pinealocytes,
closely related to the cell membrane. Further, bent plates appearing under
the form of V- and U-shaped shields were seen. Smaller lumps or spherical
masses of SB material were more often seen than in the control animal,
while parallel oriented plates as seen under a normal lighting regime were
less frequent.