Synaptic bodies (SBs) are small, rare organelles
involved in signal transduction in the retina, inner ear and pineal gland.
In vivo they undergo changes in number and size. In this study the disector,
a counting method independent of size and shape of investigated particles,
was used to verify numerical changes in vivo and to clarify whether they
occur also in vitro. Disector and single section profile counts of SBs
were performed on electron microscopic sections of chicken pineal glands
removed at day or night and cultured in an incubator for 12 hours.
In vivo significant increases of SB number, SB
profile number and SB profile length were seen from noon to midnight. Pineal
glands removed at noon and incubated for 12 hrs showed significant (p <
0.05) increases of SB number, SB profile number and length. The increase
in length was less pronounced than in vivo at 24.00, whereas the SB and
SB profile increase was more evident.
In pineal glands removed at 24.00 and incubated
for 12 hrs significant decreases of SB profile number and length and an
insignificant decrease of SB number were encountered. In vitro significantly
more SBs and SB profiles were present, the latter were insignificantly
shorter (p = 0.3) than in vivo.
Our data suggest that SB number and length in
the chicken pineal gland are subject to endogenous control, retaining their
day/night rhythm in organ culture. As the changes in number were less pronounced
in vitro than in vivo, it is assumed that in vivo the day/night rhythm
is accentuated either by the innervation of the gland or by light or by
both.
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